First wet-lab result
CPP expression & developability screen
First returned wet-lab package — SingleChain expression assay
This is an expression assay — it measures how much protein each construct produced. It is not a measurement of cell penetration, biological activity, target binding, or therapeutic efficacy.
What was observed
First returned wet-lab package: a SingleChain expression screen reporting per-construct protein yield for four CPP-panel constructs (two Protean CPP candidates + two negative controls). Three of four constructs expressed above the assay's upper quantification limit; one candidate measured 91.8 ug/mL. This is experimental evidence for the measured expression endpoint only — not cell penetration, biological activity, target binding, developability beyond yield, or clinical/therapeutic efficacy.
Candidates & controls
| Sequence | Role | Expression yield (µg/mL) | Mapping | Observation |
|---|---|---|---|---|
| PENPMKHNMKHI | Candidate | 91.8 | exact sequence ambiguous candidate id | Measured expression yield 91.8 ug/mL in this SingleChain expression screen. |
| KNHMNKEHKNQI | Candidate | >190.9> limit | exact sequence ambiguous candidate id | Expressed above the assay's upper quantification limit (>190.9 ug/mL) in this SingleChain expression screen. |
| KHNMPNPIHEK | Negative control | >186.4> limit | control not a protean candidate | Expressed above the assay's upper quantification limit (>186.4 ug/mL) in this SingleChain expression screen. |
| QKNHNEKHMKIQ | Negative control | >191.2> limit | control not a protean candidate | Expressed above the assay's upper quantification limit (>191.2 ug/mL) in this SingleChain expression screen. |
Two constructs are labelled '-neg' (negative). Their intended role and expected behaviour are NOT documented in the package, and both express at the highest reported yields. Their sequences are NOT Protean candidates. Control behaviour therefore cannot be validated from this package.
What this evidence does & does not support
Supported
- Protein expression was measurable for the tested CPP-panel constructs under the provider's SingleChain assay.
- Both Protean CPP candidates produced protein.
Not supported
- Cell penetration / cellular uptake (not measured).
- Biological activity, target binding, or efficacy (targetless; not measured).
- Developability beyond expression yield (no solubility/purity/aggregation).
- A unique Protean candidate_id linkage (sequence maps one-to-many).
- Control validation (negative-control semantics + behaviour indeterminate).
- Any comparative ranking (values are censored).
Next recommended experiment
A cellular-uptake / cell-penetration assay on the expressed candidates would be required before any cell-penetration claim; expression establishes only that the protein can be produced.
Limitations
- Experimental evidence for the measured expression endpoint only — not a claim of cell penetration, biological activity, target binding, developability beyond yield, or clinical/therapeutic efficacy.
- Three of four constructs expressed above the assay's upper quantification limit (reported as '>value'), so those values are lower bounds and no comparative ranking is possible.
- Candidate sequences match Protean's cell-penetrating-peptide design lane, but each sequence maps to many generated candidate records — no unique candidate identifier is asserted.
- The two negative-control constructs are not Protean candidates; their intended role and expected behaviour were not documented in the package, so control behaviour is not validated.
Provenance
- Run ID
- 20260713T145035Z
- Package SHA-256
- sha256:f62cd61b88fb0aa3d2de84a18b1f7ba4a18321b22d492c8408a54c3bbda55e02
- Raw package
- Preserved internally (write-protected); not public.
- Assay method
- SingleChain expression; per-construct protein yield reported in ug/mL. Three of four values are censored at the assay's upper quantification limit ('>'). No replicates, plates, QC calls, solubility/purity/aggregation, or method notes were included.